Proteintech, 67327-1-Ig, HSP20 Monoclonal antibody

Size: 20ul / 150ul The HSP20 (67327-1-Ig) by Proteintech is a Monoclonal antibody targeting HSP20 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig samples 67327-1-Ig targets HSP20 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: pig skeletal muscle tissue, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Positive IF/ICC detected in: U-118 MG cells Recommended dilution Western Blot (WB): WB : 1:2000-1:20000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:850-1:3400 Background Information Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10484 Product name: Recombinant human HSPB6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-160 aa of BC068046 Sequence: MEIPVPVQPSWLRRASAPLLGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK Predict reactive species Full Name: heat shock protein, alpha-crystallin-related, B6 Calculated Molecular Weight: 160 aa, 17 kDa Observed Molecular Weight: 17 kDa GenBank Accession Number: BC068046 Gene Symbol: HSP20 Gene ID (NCBI): 126393 RRID: AB_2882586 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14558 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.