Proteintech, 67333-1-Ig, MAP2K4 Monoclonal antibody

Size: 20ul / 150ul The MAP2K4 (67333-1-Ig) by Proteintech is a Monoclonal antibody targeting MAP2K4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 67333-1-Ig targets MAP2K4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11348 Product name: Recombinant human MAP2K4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 50-399 aa of BC036032 Sequence: NPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD Predict reactive species Full Name: mitogen-activated protein kinase kinase 4 Calculated Molecular Weight: 399 aa, 44 kDa Observed Molecular Weight: 44-50 kDa GenBank Accession Number: BC036032 Gene Symbol: MAP2K4 Gene ID (NCBI): 6416 RRID: AB_2882591 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P45985 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.