Proteintech, 67371-1-Ig, TBXAS1 Monoclonal antibody

Size: 20ul / 150ul The TBXAS1 (67371-1-Ig) by Proteintech is a Monoclonal antibody targeting TBXAS1 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, pig samples 67371-1-Ig targets TBXAS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: human peripheral blood platelets, A549 cells, human placenta tissue, pig blood Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension Background Information TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in human uterus (PMID:1386258). Specification Tested Reactivity: human, mouse, pig Cited Reactivity: mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag29177 Product name: Recombinant human TBXAS1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 178-466 aa of BC014117 Sequence: NKNRDELNGFFNKLIRNVIALRDQQAAEERRRDFLQMVLDARHSASPMGVQDFDIVRDVFSSTGCKPNPSRQHQPSPMARPLTVDEIVGQAFIFLIAGYEIITNTLSFATYLLATNPDCQEKLLREVDVFKEKHMAPEFCSLEEGLPYLDMVIAETLRMYPPAFRFTREAAQDCEVLGQRIPAGAVLEMAVGALHHDPEHWPSPETFNPERFTAEARQQHRPFTYLPFGAGPRSCLGVRLGLLEVKLTLLHVLHKFRFQACPETQVPLQLESKSALGPKNGVYIKIVSR Predict reactive species Full Name: thromboxane A synthase 1 (platelet) Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC014117 Gene Symbol: TBXAS1 Gene ID (NCBI): 6916 RRID: AB_2918487 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P24557 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.