Proteintech, 67455-1-Ig, CCT4 Monoclonal antibody

Size: 20ul / 150ul The CCT4 (67455-1-Ig) by Proteintech is a Monoclonal antibody targeting CCT4 in WB, IF/ICC, ELISA applications with reactivity to Human, Mouse, Rabbit, Pig samples 67455-1-Ig targets CCT4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rabbit, Pig samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, mouse brain tissue Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa. Specification Tested Reactivity: Human, Mouse, Rabbit, Pig Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17084 Product name: Recombinant human CCT4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 108-407 aa of BC106934 Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD Predict reactive species Full Name: chaperonin containing TCP1, subunit 4 (delta) Calculated Molecular Weight: 539 aa, 58 kDa Observed Molecular Weight: 55-58 kDa GenBank Accession Number: BC106934 Gene Symbol: CCT4 Gene ID (NCBI): 10575 RRID: AB_2935250 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P50991 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.