Proteintech, 67488-1-Ig, HRD1/SYVN1 Monoclonal antibody

Size: 20ul / 150ul The HRD1/SYVN1 (67488-1-Ig) by Proteintech is a Monoclonal antibody targeting HRD1/SYVN1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67488-1-Ig targets HRD1/SYVN1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, 4T1 cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, goat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4456 Product name: Recombinant human HRD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 242-617 aa of BC030530 Sequence: LFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPPPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGAATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH Predict reactive species Full Name: synovial apoptosis inhibitor 1, synoviolin Calculated Molecular Weight: 617 aa, 68 kDa Observed Molecular Weight: 68-76 kDa GenBank Accession Number: BC030530 Gene Symbol: HRD1 Gene ID (NCBI): 84447 RRID: AB_2882714 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q86TM6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.