Proteintech, 67550-1-Ig, EEF2 Monoclonal antibody

Size: 20ul / 150ul The EEF2 (67550-1-Ig) by Proteintech is a Monoclonal antibody targeting EEF2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 67550-1-Ig targets EEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HeLa cells, HSC-T6 cells, LoVo cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, 4T1 cells, MCF-7 cells, pig brain tissue Positive IHC detected in: human breast cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain. Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13859 Product name: Recombinant human EEF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 509-858 aa of BC126259 Sequence: VEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL Predict reactive species Full Name: eukaryotic translation elongation factor 2 Calculated Molecular Weight: 858 aa, 95 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC126259 Gene Symbol: EEF2 Gene ID (NCBI): 1938 RRID: AB_2882765 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P13639 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.