Proteintech, 67553-1-Ig, OTC Monoclonal antibody

Size: 20ul / 150ul The OTC (67553-1-Ig) by Proteintech is a Monoclonal antibody targeting OTC in WB, IHC, ELISA applications with reactivity to Human, mouse, rat, pig samples 67553-1-Ig targets OTC in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples. Tested Applications Positive WB detected in: pig liver tissue, rat liver tissue, mouse liver tissue Positive IHC detected in: human liver cancer tissue, human liver tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:4000-1:16000 Background Information Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism. Specification Tested Reactivity: Human, mouse, rat, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag24186 Product name: Recombinant human OTC protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 35-354 aa of BC074745 Sequence: VQLKGRDLLTLKNFTGEEIKYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPCFLTTQDIHLGVNESLTDTARVLSSMADAVLARVYKQSDLDTLAKEASIPIINGLSDLYHPIQILADYLTLQEHYSSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGQEEEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPQLQKPKF Predict reactive species Full Name: ornithine carbamoyltransferase Calculated Molecular Weight: 354 aa, 40 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC074745 Gene Symbol: OTC Gene ID (NCBI): 5009 RRID: AB_2882767 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P00480 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.