Proteintech, 67576-1-Ig, TGIF2 Monoclonal antibody

Size: 20ul / 150ul The TGIF2 (67576-1-Ig) by Proteintech is a Monoclonal antibody targeting TGIF2 in WB, FC (Intra), ELISA applications with reactivity to human samples 67576-1-Ig targets TGIF2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, THP-1 cells, COLO 320 cells, Ramos cells, Daudi cells, K-562 cells, Jurkat cells, HL-60 cells, MOLT-4 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information TGIF2, is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30083 Product name: Recombinant human TGIF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-237 aa of BC012816 Sequence: MSDSDLGEDEGLLSLAGKRKRRGNLPKESVKILRDWLYLHRYNAYPSEQEKLSLSGQTNLSVLQICNWFINARRRLLPDMLRKDGKDPNQFTISRRGGKASDVALPRGSSPSVLAVSVPAPTNVLSLSVCSMPLHSGQGEKPAAPFPRGELESPKPLVTPGSTLTLLTRAEAGSPTGGLFNTPPPTPPEQDKEDFSSFQLLVEVALQRAAEMELQKQQDPSLPLLHTPIPLVSENPQ Predict reactive species Full Name: TGFB-induced factor homeobox 2 Calculated Molecular Weight: 237 aa, 26 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC012816 Gene Symbol: TGIF2 Gene ID (NCBI): 60436 RRID: AB_2882789 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9GZN2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.