Proteintech, 67685-1-Ig, NF45 Monoclonal antibody

Size: 20ul / 150ul The NF45 (67685-1-Ig) by Proteintech is a Monoclonal antibody targeting NF45 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to Human, mouse, rat samples 67685-1-Ig targets NF45 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells, A431 cells, HepG2 cells, MOLT-4 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, Sp2/0 cells Positive IP detected in: HeLa cells Positive IHC detected in: human colon cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403]. Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30388 Product name: Recombinant human NF45 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-390 aa of BC000382 Sequence: MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEEPPQGEEEESMETQE Predict reactive species Full Name: interleukin enhancer binding factor 2, 45kDa Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC000382 Gene Symbol: ILF2 Gene ID (NCBI): 3608 RRID: AB_2882878 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q12905 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.