Proteintech, 67736-1-Ig, PTGES3 Monoclonal antibody

Size: 20ul / 150ul The PTGES3 (67736-1-Ig) by Proteintech is a Monoclonal antibody targeting PTGES3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 67736-1-Ig targets PTGES3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, PC-3 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766). Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7870 Product name: Recombinant human PTGES3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-160 aa of BC003005 Sequence: MQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRTDRSILCCLRKGESGQSWPRLTKERAKLNWLSVDFNNWKDWEDDSDEDMSNFDRFSEMMNNMGGDEDVDLPEVDGADDDSQDSDDEKMPDLE Predict reactive species Full Name: prostaglandin E synthase 3 (cytosolic) Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: BC003005 Gene Symbol: PTGES3 Gene ID (NCBI): 10728 RRID: AB_2918505 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q15185 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.