Proteintech, 67741-1-Ig, SIX1 Monoclonal antibody

Size: 20ul / 150ul The SIX1 (67741-1-Ig) by Proteintech is a Monoclonal antibody targeting SIX1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67741-1-Ig targets SIX1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: L02 cells, HeLa cells, rat skeletal muscle tissue, mouse skeletal muscle tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28570 Product name: Recombinant human SIX1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-284 aa of BC008874 Sequence: MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLCGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDLGS Predict reactive species Full Name: SIX homeobox 1 Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 30-35 kDa GenBank Accession Number: BC008874 Gene Symbol: SIX1 Gene ID (NCBI): 6495 RRID: AB_2918510 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q15475 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.