Proteintech, 67768-1-Ig, ALCAM Monoclonal antibody

Size: 20ul / 150ul The ALCAM (67768-1-Ig) by Proteintech is a Monoclonal antibody targeting ALCAM in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications with reactivity to human samples 67768-1-Ig targets ALCAM in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-1080 cells, U2OS cells, A549 cells, LNCaP cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human breast cancer tissue Positive IF-Fro detected in: mouse breast cancer Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16892 Product name: Recombinant human ALCAM protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 28-400 aa of BC137097 Sequence: WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVE Predict reactive species Full Name: activated leukocyte cell adhesion molecule Calculated Molecular Weight: 583 aa, 65 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC137097 Gene Symbol: ALCAM Gene ID (NCBI): 214 RRID: AB_2918534 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q13740 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.