Proteintech, 67803-1-Ig, HK3 Monoclonal antibody

Size: 20ul / 150ul The HK3 (67803-1-Ig) by Proteintech is a Monoclonal antibody targeting HK3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig samples 67803-1-Ig targets HK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: Raji cells, Ramos cells, RAW 264.7 cells, human saliva, rat spleen tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30798 Product name: Recombinant human HK3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 622-923 aa of BC028129 Sequence: LLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV Predict reactive species Full Name: hexokinase 3 (white cell) Calculated Molecular Weight: 923 aa, 99 kDa Observed Molecular Weight: 99 kDa GenBank Accession Number: BC028129 Gene Symbol: HK3 Gene ID (NCBI): 3101 RRID: AB_2918566 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P52790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.