Proteintech, 67811-1-Ig, ALKBH5 Monoclonal antibody

Size: 20ul / 150ul The ALKBH5 (67811-1-Ig) by Proteintech is a Monoclonal antibody targeting ALKBH5 in WB, IHC, ELISA applications with reactivity to Human, Mouse, Rat samples 67811-1-Ig targets ALKBH5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: 4T1 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa. Specification Tested Reactivity: Human, Mouse, Rat Cited Reactivity: human, mouse, rat, monkey, hamster Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10490 Product name: Recombinant human ALKBH5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 57-383 aa of BC062339 Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG Predict reactive species Full Name: alkB, alkylation repair homolog 5 (E. coli) Calculated Molecular Weight: 458 aa, 51 kDa Observed Molecular Weight: 40-44 kDa GenBank Accession Number: BC062339 Gene Symbol: ALKBH5 Gene ID (NCBI): 54890 RRID: AB_2918574 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6P6C2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.