Proteintech, 67812-1-Ig, E2F4 Monoclonal antibody

Size: 20ul / 150ul The E2F4 (67812-1-Ig) by Proteintech is a Monoclonal antibody targeting E2F4 in WB, ELISA applications with reactivity to human, mouse, rat samples 67812-1-Ig targets E2F4 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, HSC-T6 cells, RKO cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, FaDu cells, SiHa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28533 Product name: Recombinant human E2F4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-413 aa of BC033180 Sequence: MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL Predict reactive species Full Name: E2F transcription factor 4, p107/p130-binding Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC033180 Gene Symbol: E2F4 Gene ID (NCBI): 1874 RRID: AB_2918575 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q16254 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.