Proteintech, 67835-1-Ig, ELAVL4 Monoclonal antibody

Size: 20ul / 150ul The ELAVL4 (67835-1-Ig) by Proteintech is a Monoclonal antibody targeting ELAVL4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67835-1-Ig targets ELAVL4 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: pig brain tissue, U2OS cells, SH-SY5Y cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, rat cerebellum tissue, mouse cerebellum tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 ) Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: mouse, rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag3678 Product name: Recombinant human ELAVL4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC036071 Sequence: MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVGQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAITSLNGYRLGDRVLQVSFKTNKAHKS Predict reactive species Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) Calculated Molecular Weight: 380 aa, 42 kDa Observed Molecular Weight: 32-40 kDa GenBank Accession Number: BC036071 Gene Symbol: ELAVL4 Gene ID (NCBI): 1996 RRID: AB_2918597 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P26378 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.