Proteintech, 67949-1-Ig, HDAC11 Monoclonal antibody

Size: 20ul / 150ul The HDAC11 (67949-1-Ig) by Proteintech is a Monoclonal antibody targeting HDAC11 in WB, IHC, ELISA applications with reactivity to Human samples 67949-1-Ig targets HDAC11 in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, SKOV-3 cells, T-47D cells Positive IHC detected in: human kidney tissue, human colon cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC11 belongs to Class IV HDACs. HDAC11 is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants. HDAC11 has 2 isoforms with the molecular mass of 33 and 39 kDa. Specification Tested Reactivity: Human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28335 Product name: Recombinant human HDAC11 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-347 aa of BC009676 Sequence: MLHTTQLYQHVPETRWPIVYSPRYNITFMGLEKLHPFDAGKWGKVINFLKEEKLLSDSMLVEAREASEEDLLVVHTRRYLNELKWSFAVATITEIPPVIFLPNFLVQRKVLRPLRTQTGGTIMAGKLAVERGWAINVGGGFHHCSSDRGGGFCAYADITLAIKFLFERVEGISRATIIDLDAHQGNGHERDFMDDKRVYIMDVYNRHIYPGDRFAKQAIRRKVELEWGTEDDEYLDKVERNIKKSLQEHLPDVVVYNAGTDILEGDRLGGLSISPAGIVKRDELVFRMVRGRRVPILMVTSGGYQKRTARIIADSILNLFGLGLIGPESPSVSAQNSDTPLLPPAVP Predict reactive species Full Name: histone deacetylase 11 Calculated Molecular Weight: 347 aa, 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC009676 Gene Symbol: HDAC11 Gene ID (NCBI): 79885 RRID: AB_2918701 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q96DB2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.