Proteintech, 67995-1-Ig, Gephyrin/GPHN Monoclonal antibody

Size: 20ul / 150ul The Gephyrin/GPHN (67995-1-Ig) by Proteintech is a Monoclonal antibody targeting Gephyrin/GPHN in WB, ELISA applications with reactivity to human samples 67995-1-Ig targets Gephyrin/GPHN in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag23708 Product name: Recombinant human GPHN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-315 aa of BC030016 Sequence: MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQDPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKEVIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILPALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEEKKDSGVASTEDSSSSHITAAAIAAKKHPFYTSPAVVMAHGEQPIPGLINYSHHSTDERIPDSIISRGVQVLPRDTASLSTTPSESPRAQATSRLSTA Predict reactive species Full Name: gephyrin Calculated Molecular Weight: 769 aa, 83 kDa Observed Molecular Weight: 93 kDa GenBank Accession Number: BC030016 Gene Symbol: GPHN Gene ID (NCBI): 10243 RRID: AB_2918744 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9NQX3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.