Proteintech, 68000-1-Ig, Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal antibody

Size: 20ul / 100ul The Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) (68000-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, FC (Intra), ELISA applications with reactivity to human samples 68000-1-Ig targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: TF-1 cells, GM-CSF treated TF-1 cells Positive FC (Intra) detected in: GM-CSF treated TF-1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 5A Calculated Molecular Weight: 794 aa, 92 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC027036 Gene Symbol: STAT5A Gene ID (NCBI): 6776 RRID: AB_2918748 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P42229 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.