Proteintech, 68136-1-Ig, Phospho-Caspase 9 (Thr125) Monoclonal antibody

Size: 20ul / 100ul The Phospho-Caspase 9 (Thr125) (68136-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-Caspase 9 (Thr125) in WB, ELISA applications with reactivity to human, rat samples 68136-1-Ig targets Phospho-Caspase 9 (Thr125) in WB, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, Calyculin A treated Jurkat cells, Calyculin A treated HSC-T6 cells, Calyculin A treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46 kDa precursor protein which can be cleaved into a 35 kDa subunit and a 11 kDa subunit. Control of all caspases is tightly regulated by a series of phosphorylation events enacted by several different kinases. Caspase-9 is the most heavily phosphorylated of all caspases, with phosphorylation of at least 11 distinct residues in all three caspase-9 domains by nine kinases. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507, PMID: 29066624) Specification Tested Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 9, apoptosis-related cysteine peptidase Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC002452 Gene Symbol: Caspase 9 Gene ID (NCBI): 842 RRID: AB_2923663 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P55211 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.