Proteintech, 68147-1-Ig, ENO3 Monoclonal antibody

Size: 20ul / 150ul The ENO3 (68147-1-Ig) by Proteintech is a Monoclonal antibody targeting ENO3 in WB, IF/ICC, ELISA applications with reactivity to Human, Mouse, Rat, Pig samples 68147-1-Ig targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig skeletal muscle tissue, rabbit skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, mouse brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693) Specification Tested Reactivity: Human, Mouse, Rat, Pig Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9642 Product name: Recombinant human ENO3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 94-434 aa of BC017249 Sequence: MIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK Predict reactive species Full Name: enolase 3 (beta, muscle) Calculated Molecular Weight: 434 aa, 47 kDa Observed Molecular Weight: 42-47 kDa GenBank Accession Number: BC017249 Gene Symbol: ENO3 Gene ID (NCBI): 2027 RRID: AB_2923671 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13929 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.