Proteintech, 68157-1-Ig, HIBCH Monoclonal antibody

Size: 20ul / 150ul The HIBCH (68157-1-Ig) by Proteintech is a Monoclonal antibody targeting HIBCH in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 68157-1-Ig targets HIBCH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: HepG2 cells, Jurkat cells, K-562 cells, pig heart tissue, rabbit heart tissue, rat heart tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301). Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6473 Product name: Recombinant human HIBCH protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-386 aa of BC067822 Sequence: MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLGKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF Predict reactive species Full Name: 3-hydroxyisobutyryl-Coenzyme A hydrolase Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: BC067822 Gene Symbol: HIBCH Gene ID (NCBI): 26275 RRID: AB_2935261 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6NVY1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.