Proteintech, 68161-1-Ig, CACYBP Monoclonal antibody

Size: 20ul / 150ul The CACYBP (68161-1-Ig) by Proteintech is a Monoclonal antibody targeting CACYBP in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 68161-1-Ig targets CACYBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HCT 116 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2340 Product name: Recombinant human CACYBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-228 aa of BC022352 Sequence: MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAKGDTEF Predict reactive species Full Name: calcyclin binding protein Calculated Molecular Weight: 228 aa, 26 kDa Observed Molecular Weight: 26-30 kDa GenBank Accession Number: BC022352 Gene Symbol: CACYBP Gene ID (NCBI): 27101 RRID: AB_2923680 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9HB71 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.