Proteintech, 68299-1-Ig, LEPREL2 Monoclonal antibody

Size: 20ul / 150ul The LEPREL2 (68299-1-Ig) by Proteintech is a Monoclonal antibody targeting LEPREL2 in WB, IHC, ELISA applications with reactivity to Human, Rat, Mouse samples 68299-1-Ig targets LEPREL2 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples. Tested Applications Positive WB detected in: rat testis tissue, HeLa cells Positive IHC detected in: mouse ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V. Specification Tested Reactivity: Human, Rat, Mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8850 Product name: Recombinant human LEPREL2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 223-605 aa of BC017217 Sequence: RALNQYQAQLGEPRPGLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDPWTPAALIPEALREKLREDQEKRPWDHEPVKPKPLTYWKDVLLLEGVTLTQDSRQLNGSERAVLDGLLTPAECGVLLQLAKDAAGAGARSGYRGRRSPHTPHERFEGLTVLKAAQLARAGTVGSQGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLVCRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSGLLYLNDDFQGGDLFFTEPNALTVTARVRPRCGRLVAFSSGVENPHGVWAVTRGRRCALALWHTWAPEHREQEWIEAKELLQESQEEEEEEEEEMPSKDPSPEPPSRRHQRVQDKTGRAPRVREEL Predict reactive species Full Name: leprecan-like 2 Calculated Molecular Weight: 736 aa, 82 kDa Observed Molecular Weight: 82 kDa GenBank Accession Number: BC017217 Gene Symbol: LEPREL2 Gene ID (NCBI): 10536 RRID: AB_2935379 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q8IVL6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.