Proteintech, 68318-1-Ig, NFIC Monoclonal antibody

Size: 20ul / 150ul The NFIC (68318-1-Ig) by Proteintech is a Monoclonal antibody targeting NFIC in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 68318-1-Ig targets NFIC in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HSC-T6 cells, Saos-2 cells, U2OS cellls, LNCaP cells, HeLa cells, NIH/3T3 cells, 4T1 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9472 Product name: Recombinant human NFIC protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-351 aa of BC012120 Sequence: MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSSRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQ Predict reactive species Full Name: nuclear factor I/C (CCAAT-binding transcription factor) Calculated Molecular Weight: 428aa,48 kDa; 508aa,57 kDa Observed Molecular Weight: 50-56 kDa GenBank Accession Number: BC012120 Gene Symbol: NFIC Gene ID (NCBI): 4782 RRID: AB_3085054 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08651 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.