Proteintech, 68410-1-Ig, SYAP1 Monoclonal antibody

Size: 20ul / 150ul The SYAP1 (68410-1-Ig) by Proteintech is a Monoclonal antibody targeting SYAP1 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 68410-1-Ig targets SYAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U2OS cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Positive IP detected in: L02 cells Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9626 Product name: Recombinant human SYAP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-352 aa of BC014657 Sequence: MFRGLSSWLGLQQPVAGGGQPNGDALPEQPSETVAESAEEELQQAGDQELLHQAKDFGNYLFNFASAATKKITESVAETAQTIKKSVEEGKIDGIIDKTIIGDFQKEQKKFVEEQHTKKSEAAVPPWVDTNDEETIQQQILALSADKRNFLRDPPAGVQFNFDFDQMYPVALVMLQEDELLSKMRFALVPKLVKEEVFWRNYFYRVSLIKQSAQLTALAAQQQAAGKEEKSNGREQDLPLAEAVRPKTPPVVIKSQLKTQEDEEEISTSPGVSEFVSDAFDACNLNQEDLRKEMEQLVLDKKQEETAVLEEDSADWEKELQQELQEYEVVTESEKRDENWDKEIEKMLQEEN Predict reactive species Full Name: synapse associated protein 1, SAP47 homolog (Drosophila) Calculated Molecular Weight: 352 aa, 40 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC014657 Gene Symbol: SYAP1 Gene ID (NCBI): 94056 RRID: AB_3085127 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96A49 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.