Proteintech, 68425-1-Ig, ATP6V1H Monoclonal antibody

Size: 20ul / 150ul The ATP6V1H (68425-1-Ig) by Proteintech is a Monoclonal antibody targeting ATP6V1H in WB, IF/ICC, ELISA applications with reactivity to Human, mouse, rat, rabbit, pig samples 68425-1-Ig targets ATP6V1H in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples. Tested Applications Positive WB detected in: U2OS cells, pig brain tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. Specification Tested Reactivity: Human, mouse, rat, rabbit, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10130 Product name: Recombinant human ATP6V1H protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 136-483 aa of BC025275 Sequence: FTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS Predict reactive species Full Name: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H Calculated Molecular Weight: 483 aa, 56 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC025275 Gene Symbol: ATP6V1H Gene ID (NCBI): 51606 RRID: AB_2943428 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UI12 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.