Proteintech, 68473-1-Ig, MEN1 Monoclonal antibody

Size: 20ul / 150ul The MEN1 (68473-1-Ig) by Proteintech is a Monoclonal antibody targeting MEN1 in WB, IHC, ELISA applications with reactivity to human samples 68473-1-Ig targets MEN1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, U2OS cells, LNCaP cells, K-562 cells, Jurkat cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. It is located in nucleus and ubiquitous expression in spleen and lymph node. The calculated molecular weight of MEN1 is 68 kDa. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7566 Product name: Recombinant human MEN1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 226-575 aa of BC002544 Sequence: QKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPAASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL Predict reactive species Full Name: multiple endocrine neoplasia I Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 68-70 kDa GenBank Accession Number: BC002544 Gene Symbol: MEN1 Gene ID (NCBI): 4221 RRID: AB_3085184 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O00255 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.