Proteintech, 68505-1-Ig, ATP1A1/2 Monoclonal antibody

Size: 20ul / 150ul The ATP1A1/2 (68505-1-Ig) by Proteintech is a Monoclonal antibody targeting ATP1A1/2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 68505-1-Ig targets ATP1A1/2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: rat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue Positive IF/ICC detected in: C2C12 cells, HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2. Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10489 Product name: Recombinant human ATP1A2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 347-700 aa of BC052271 Sequence: KRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALSRIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSIHEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSGKFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPMSQVNPREAKACVVHGSDLKDMTSEQLDEILKNHTEIVFARTSPQQKLIIVE Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide Calculated Molecular Weight: 1020 aa, 112 kDa Observed Molecular Weight: 97-100 kDa GenBank Accession Number: BC052271 Gene Symbol: ATP1A2 Gene ID (NCBI): 477 RRID: AB_3085215 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P50993 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.