Proteintech, 68512-1-Ig, IGFBP5 Monoclonal antibody

Size: 20ul / 150ul The IGFBP5 (68512-1-Ig) by Proteintech is a Monoclonal antibody targeting IGFBP5 in WB, IHC, ELISA applications with reactivity to Human, mouse samples 68512-1-Ig targets IGFBP5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: T-47D cells, SKOV-3 cells, Saos-2 cells, U2OS cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5. Specification Tested Reactivity: Human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag33797 Product name: Recombinant human IGFBP5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-272 aa of BC011453 Sequence: MVLLTAVLLLLAAYAGPAQSLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE Predict reactive species Full Name: insulin-like growth factor binding protein 5 Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 30-33 kDa GenBank Accession Number: BC011453 Gene Symbol: IGFBP5 Gene ID (NCBI): 3488 RRID: AB_3085221 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P24593 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.