Proteintech, 68592-1-Ig, AEG-1/MTDH Monoclonal antibody

Size: 20ul / 150ul The AEG-1/MTDH (68592-1-Ig) by Proteintech is a Monoclonal antibody targeting AEG-1/MTDH in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 68592-1-Ig targets AEG-1/MTDH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, MCF-10A cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells Positive IHC detected in: human rectal cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5033 Product name: Recombinant human AEG-1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 233-582 aa of BC045642 Sequence: EQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKAEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET Predict reactive species Full Name: metadherin Calculated Molecular Weight: 582 aa, 64 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC045642 Gene Symbol: AEG-1/MTDH Gene ID (NCBI): 92140 RRID: AB_3085291 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q86UE4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.