Proteintech, 68632-1-Ig, TFEB Monoclonal antibody

Size: 20ul / 150ul The TFEB (68632-1-Ig) by Proteintech is a Monoclonal antibody targeting TFEB in WB, ELISA applications with reactivity to Human samples 68632-1-Ig targets TFEB in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: SK-BR-3 cells, HepG2 cells, Saos-2 cells, Ramos cells, Raji cells, Daudi cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200). Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag24850 Product name: Recombinant human TFEB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-476 aa of BC032448 Sequence: MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL Predict reactive species Full Name: transcription factor EB Calculated Molecular Weight: 476 aa, 53 kDa Observed Molecular Weight: 53, 65-70 kDa GenBank Accession Number: BC032448 Gene Symbol: TFEB Gene ID (NCBI): 7942 RRID: AB_3085323 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P19484 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.