Proteintech, 68637-1-Ig, RAD1 Monoclonal antibody

Size: 20ul / 150ul The RAD1 (68637-1-Ig) by Proteintech is a Monoclonal antibody targeting RAD1 in WB, FC (Intra), IP, ELISA applications with reactivity to human samples 68637-1-Ig targets RAD1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MKN-45 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, Daudi cells, K-562 cells Positive IP detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag33257 Product name: Recombinant human RAD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-282 aa of BC006837 Sequence: MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES Predict reactive species Full Name: RAD1 homolog (S. pombe) Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC006837 Gene Symbol: RAD1 Gene ID (NCBI): 5810 RRID: AB_3085326 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60671 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.