Proteintech, 68642-1-Ig, COG1 Monoclonal antibody

Size: 20ul / 150ul The COG1 (68642-1-Ig) by Proteintech is a Monoclonal antibody targeting COG1 in WB, IF/ICC, ELISA applications with reactivity to human, rabbit samples 68642-1-Ig targets COG1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples. Tested Applications Positive WB detected in: HepG2 cells, LNCaP cells, Jurkat cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452). Specification Tested Reactivity: human, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag33924 Product name: Recombinant human COG1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 561-980 aa of BC021985 Sequence: ADAGTVQEMLRTQSVACIKHIVDCIRAELQSIEEGVQGQQDALNSAKLHSVLFMARLCQSLGELCPHLKQCILGKSESSEKPAREFRALRKQGKVKTQEIIPTQAKWQEVKEVLLQQSVMGYQVWSSAVVKVLIHGFTQSLLLDDAGSVLATATSWDELEIQEEAESGSSVTSKIRLPAQPSWYVQSFLFSLCQEINRVGGHALPKVTLQEMLKSCMVQVVAAYEKLSEEKQIKKEGAFPVTQNRALQLLYDLRYLNIVLTAKGDEVKSGRSKPDSRIEKVTDHLEALIDPFDLDVFTPHLNSNLHRLVQRTSVLFGLVTGTENQLAPRSSTFNSQEPHNILPLASSQIRFGLLPLSMTSTRKAKSTRNIETKAQVVPPARSTAGDPTVPGSLFRQLVSEEDNTSAPSLFKLGWLSSMTK Predict reactive species Full Name: component of oligomeric golgi complex 1 Calculated Molecular Weight: 109 kDa Observed Molecular Weight: 100-109 kDa GenBank Accession Number: BC021985 Gene Symbol: COG1 Gene ID (NCBI): 9382 RRID: AB_3670386 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q8WTW3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.