Proteintech, 68653-1-Ig, CEACAM1/CD66a Monoclonal antibody

Size: 20ul / 150ul The CEACAM1/CD66a (68653-1-Ig) by Proteintech is a Monoclonal antibody targeting CEACAM1/CD66a in WB, IF/ICC, FC, ELISA applications with reactivity to human samples 68653-1-Ig targets CEACAM1/CD66a in WB, IF/ICC, FC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HT-29 cells Positive IF/ICC detected in: HT-29 cells Positive FC detected in: HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) Calculated Molecular Weight: 526 aa, 58 kDa Observed Molecular Weight: 120-150 kDa GenBank Accession Number: BC014473 Gene Symbol: CEACAM1 Gene ID (NCBI): 634 RRID: AB_3670395 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P13688 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.