Proteintech, 68709-1-Ig, NPTX2 Monoclonal antibody

Size: 20ul / 150ul The NPTX2 (68709-1-Ig) by Proteintech is a Monoclonal antibody targeting NPTX2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 68709-1-Ig targets NPTX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: pig pituitary gland tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068) Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag33808 Product name: Recombinant human NPTX2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC009924 Sequence: MLALLAASVALAVAAGAQDSPAPGSRFVCTALPPEAVHAGCPLPAMPMQGGAQSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGGKARGAGATGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVSNAGLPGDFREVLQQRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTESTLNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAFTICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVL Predict reactive species Full Name: neuronal pentraxin II Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC009924 Gene Symbol: NPTX2 Gene ID (NCBI): 4885 RRID: AB_3670402 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P47972 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.