Proteintech, 68735-2-Ig, ATP1A1 Monoclonal antibody

Size: 20ul / 150ul The ATP1A1 (68735-2-Ig) by Proteintech is a Monoclonal antibody targeting ATP1A1 in WB, IHC, ELISA applications with reactivity to human samples 68735-2-Ig targets ATP1A1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: unboiled A431 cells, unboiled HepG2 cells Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5990 Product name: Recombinant human ATP1A1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 347-681 aa of BC050359 Sequence: TAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDI Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide Calculated Molecular Weight: 113 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC050359 Gene Symbol: ATP1A1 Gene ID (NCBI): 476 ENSEMBL Gene ID: ENSG00000163399 RRID: AB_3670420 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05023 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.