Proteintech, 68803-2-Ig, FOXP3 Monoclonal antibody

Size: 20ul / 150ul The FOXP3 (68803-2-Ig) by Proteintech is a Monoclonal antibody targeting FOXP3 in WB, IHC, ELISA applications with reactivity to human samples 68803-2-Ig targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MJ cells Positive IHC detected in: human tonsillitis tissue, human appendicitis tissue, human lymphoma tissue, human ovary cancer tissue, human rectal cancer tissue, MJ cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag23089 Product name: Recombinant human FOXP3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 107-196 aa of BC113401 Sequence: STVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLAN Predict reactive species Full Name: forkhead box P3 Calculated Molecular Weight: 431 aa, 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC113401 Gene Symbol: FOXP3 Gene ID (NCBI): 50943 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9BZS1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.