Proteintech, 68811-1-Ig, CHMP4B Monoclonal antibody

Size: 20ul / 150ul The CHMP4B (68811-1-Ig) by Proteintech is a Monoclonal antibody targeting CHMP4B in WB, IF/ICC, ELISA applications with reactivity to Human samples 68811-1-Ig targets CHMP4B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells Positive IF/ICC detected in: HCT 116 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837). Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4544 Product name: Recombinant human CHMP4B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC033859 Sequence: MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM Predict reactive species Full Name: chromatin modifying protein 4B Calculated Molecular Weight: 224 aa, 25 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC033859 Gene Symbol: CHMP4B Gene ID (NCBI): 128866 RRID: AB_3670432 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9H444 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.