Proteintech, 80008-1-RR, Alpha smooth muscle actin specific Recombinant monoclonal antibody

Size: 20ul / 100ul The Alpha smooth muscle actin specific (80008-1-RR) by Proteintech is a Recombinant antibody targeting Alpha smooth muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig, chicken, zebrafish samples 80008-1-RR targets Alpha smooth muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples. Tested Applications Positive WB detected in: mouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine tissue, rat colon tissue Positive IHC detected in: human liver tissue, human colon tissue, mouse colon tissue, rat colon tissue, human heart tissue, human appendicitis tissue, human rectal cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue, mouse embryo tissue Positive IF-Fro detected in: mouse small intestine tissue Positive FC (Intra) detected in: C2C12 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:2500-1:10000 Immunofluorescence (IF)-P: IF-P : 1:500-1:2000 Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. Specification Tested Reactivity: human, mouse, rat, pig, chicken, zebrafish Cited Reactivity: human, mouse, rat, pig, duck Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: actin, alpha 2, smooth muscle, aorta Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: NM_001613 Gene Symbol: Alpha smooth muscle actin Gene ID (NCBI): 59 RRID: AB_2882938 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P62736 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.