Proteintech, 80024-1-RR, Phospho-JNK (Tyr185) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-JNK (Tyr185) (80024-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-JNK (Tyr185) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 80024-1-RR targets Phospho-JNK (Tyr185) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, Calyculin A treated HEK-293T cells, UV treated HEK-293T cells, UV treated NIH/3T3 cells, Anisomycin treated NIH/3T3 cells Positive IF/ICC detected in: UV treated HEK-293 cells Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, zebrafish, sheep Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase 8 Observed Molecular Weight: 37-46 kDa GenBank Accession Number: NM_139049 Gene Symbol: JNK Gene ID (NCBI): 5599 RRID: AB_2882943 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P45983 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.