Proteintech, 80031-1-RR, Phospho-ERK1/2 (Thr202/Tyr204) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-ERK1/2 (Thr202/Tyr204) (80031-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-ERK1/2 (Thr202/Tyr204) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 80031-1-RR targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, Calyculin A treated NIH/3T3 cells, PC-3 cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells Positive FC (Intra) detected in: Calyculin A treated HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension Background Information Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. This antibody simultaneously recognizes the phosphorylation of ERK1 at Thr202 and Tyr204, as well as the phosphorylation of ERK2 at Thr185 and Tyr187. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase 3 Calculated Molecular Weight: 38-43 kDa Observed Molecular Weight: 38-40 kDa GenBank Accession Number: NM_002746 Gene Symbol: ERK1 Gene ID (NCBI): 5595 RRID: AB_2918858 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P27361 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.