Proteintech, 80059-1-RR, Phospho-EIF4B (Ser406) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EIF4B (Ser406) (80059-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EIF4B (Ser406) in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 80059-1-RR targets Phospho-EIF4B (Ser406) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, Calyculin A treated HEK-293T cells, PC-3 cells, Calyculin A treated PC-3 cells, Calyculin A treated Hela cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells Positive IHC detected in: Jurkat cells, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Background Information EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 4B Calculated Molecular Weight: 611 aa, 69 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC073154 Gene Symbol: EIF4B Gene ID (NCBI): 1975 RRID: AB_2918859 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P23588 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.