Proteintech, 80084-1-RR, Phospho-Beta Catenin (Ser675) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Beta Catenin (Ser675) (80084-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Beta Catenin (Ser675) in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 80084-1-RR targets Phospho-Beta Catenin (Ser675) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, λ phosphatase treated HEK-293, λ phosphatase treated NIH/3T3 cells Positive IF/ICC detected in: λ phosphatase treated MCF-7 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: catenin (cadherin-associated protein), beta 1, 88kDa Calculated Molecular Weight: 781 aa, 86 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC058926 Gene Symbol: Beta Catenin Gene ID (NCBI): 1499 ENSEMBL Gene ID: ENSG00000168036 RRID: AB_2918863 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35222 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.