Proteintech, 80108-1-RR, Phospho-RPS6KA1 (Ser380) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-RPS6KA1 (Ser380) (80108-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-RPS6KA1 (Ser380) in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 80108-1-RR targets Phospho-RPS6KA1 (Ser380) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PDGF treated NIH/3T3 cells, HeLa cells, Jurkat cells, Calyculin A treated HeLa cells, Calyculin A treated Jurkat cells Positive IHC detected in: human malignant melanoma tissue, human placenta tissue, Jurkat cells, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: TPA treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1 Calculated Molecular Weight: 735 aa, 83 kDa Observed Molecular Weight: 83-90 kDa GenBank Accession Number: BC014966 Gene Symbol: RPS6KA1 Gene ID (NCBI): 6195 RRID: AB_2918865 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15418 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.