Proteintech, 80127-1-RR, Phospho-STK11/LKB1 (Thr189) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-STK11/LKB1 (Thr189) (80127-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-STK11/LKB1 (Thr189) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 80127-1-RR targets Phospho-STK11/LKB1 (Thr189) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PC-3 cells, HEK-293 cells, NIH/3T3 cells, Calyculin A treated HEK-293 cells, Calyculin A treated PC-3 cells, Calyculin A treated NIH/3T3 cells Positive IF/ICC detected in: Calyculin A treated PC-3 cells Positive FC (Intra) detected in: Calyculin A treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: serine/threonine kinase 11 Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC007981 Gene Symbol: LKB1 Gene ID (NCBI): 6794 RRID: AB_2918867 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15831 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.