Proteintech, 80149-1-RR, STAT3 Recombinant monoclonal antibody

Size: 20ul / 100ul The STAT3 (80149-1-RR) by Proteintech is a Recombinant antibody targeting STAT3 in WB, IHC, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 80149-1-RR targets STAT3 in WB, IHC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, Neuro-2a cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive ChIP-qPCR detected in: IL-6 (100 ng/ml, 30 min) HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 3 (acute-phase response factor) Calculated Molecular Weight: 770 aa, 88 kDa Observed Molecular Weight: 88 kDa GenBank Accession Number: BC000627 Gene Symbol: STAT3 Gene ID (NCBI): 6774 RRID: AB_2918869 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P40763 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.