Proteintech, 80219-1-RR, Mono/Di-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody

Size: 20ul / 100ul The Mono/Di-Methyl-Histone H3 (Lys9) (80219-1-RR) by Proteintech is a Recombinant antibody targeting Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat samples 80219-1-RR targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: THP-1 cells, K-562 cells, Jurkat cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells, PC-12 cells Positive IF/ICC detected in: HeLa cells Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:5000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15-18 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 RRID: AB_2918878 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.