Proteintech, 80257-1-RR, Phospho-SMAD1 (Ser195) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-SMAD1 (Ser195) (80257-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-SMAD1 (Ser195) in WB, ELISA, ChIP-qPCR applications with reactivity to human samples 80257-1-RR targets Phospho-SMAD1 (Ser195) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BMP2 treated HepG2 cells, A431 cells, UV treated A431 cells Positive ChIP-qPCR detected in: BMP2-HepG2 (50 ng/mL, 1 h) HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SMAD family member 1 Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC001878 Gene Symbol: SMAD1 Gene ID (NCBI): 4086 RRID: AB_2918881 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15797 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.