Proteintech, 80340-1-RR, Phospho-Hamartin/TSC1 (Ser511) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Hamartin/TSC1 (Ser511) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 80340-1-RR targets Phospho-Hamartin/TSC1 (Ser511) in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells Positive FC (Intra) detected in: Calyculin A treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205). Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tuberous sclerosis 1 Calculated Molecular Weight: 130 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: NM_000368 Gene Symbol: Hamartin/TSC1 Gene ID (NCBI): 7248 RRID: AB_2918889 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q92574 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.